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July 23, 2015
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This protocol describes a procedure for enriching ECM proteins from tissues or tumors and deglycosylating and digesting the ECM-enriched preparations into peptides to analyze their protein composition by mass spectrometry.
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Cite this Article
Naba, A., Clauser, K. R., Hynes, R. O. Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis. J. Vis. Exp. (101), e53057, doi:10.3791/53057 (2015).
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