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JoVE Journal Bioengineering
Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
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Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

Article DOI: 10.3791/53078-v • 09:56 min • September 29th, 2015
September 29th, 2015 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, 2Shriners Hospitals for Children-Boston

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Summary
Automatic Translation

The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.

Tags

Microfluidic Devices Collagen Deposition Microtissue Stabilization 3D Culture Cell Morphology Cell Function Microfluidic Tissue Models Collagen Type I Gels Layer-by-layer Deposition Ultrathin Collagen Assemblies Primary Hepatocytes Long Term Culture
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