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Anvendelse af Ramsay-analysen til at måle Fluid sekretion og Ion Flux priser i
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Anvendelse af Ramsay-analysen til at måle Fluid sekretion og Ion Flux priser i

Article DOI: 10.3791/53144-v • 13:30 min • November 25th, 2015
November 25th, 2015 •
1, 1
1Department of Internal Medicine, University of Texas Southwestern Medical Center

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Denne protokol beskriver anvendelsen af Ramsay assay til måling af væske sekretionshastigheder fra isolerede Malpighian (renal) tubuli fra Drosophila melanogaster. Desuden anvendelsen af ​​ionspecifikke elektroder måler natrium og kalium koncentrationer i den udskilte væske, tillader beregning af transepitel ion flux, er beskrevet.

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Cellular Biology Ramsay assay ion-specifikke elektrode renal tubulus Malpighian tubulus, Fluid sekretion kalium flux natrium flux epithelial ion transport renal fysiologi
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