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Imaging Intracellulær Trafficking of APP med Photoactivatable GFP
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Imaging Intracellulær Trafficking of APP med Photoactivatable GFP

Article DOI: 10.3791/53153-v • 07:55 min • October 17th, 2015
October 17th, 2015 •
1, 1,2
1Department of Physiology and Pharmacology, Robarts Research Institute, Western University, 2Department of Clinical Neurological Sciences, Western University

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Mens transport av celleoverflateproteiner som er forholdsvis lett studert, visualisering handel med intracellulære proteiner er mye vanskeligere. Her bruker vi konstruerer innlemme photoactivatable GFP og vise en metode for å nøyaktig følge amyloidforløperprotein fra Golgi-apparatet til nedstrøms avdelinger og følge sin klarering.

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Cellular Biology amyloidforløperprotein Photo-aktiverbart GFP Golgi lysosomer Intracellulær trafficking Alzheimers sykdom β-amyloid Live-celle mikros
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