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Optisk Kortlægning af Intra-reticulum Ca

Article DOI: 10.3791/53166-v • 09:26 min • September 10th, 2015
September 10th, 2015 •
1, 2, 1
1Department of Pharmacology, University of California Davis, 2Departments of Pharmacology and Biomedical Engineering, University of California Davis

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Denne artikel beskriver den detaljerede protokol og nødvendigt udstyr til dobbelt optisk kortlægning af transmembranpotentiale (V m) og gratis intra-reticulum (SR) Ca2 + i Langendorff-perfunderet kanin hjerte. Denne metode giver mulighed for direkte observation og kvantificering af V m og SR Ca 2+ dynamik i den intakte hjerte.

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Molecular Biology optiske kortlægning reticulum calcium calcium-sensitive farvestof spændingsfølsomme farvestof arytmi hjerteelektrofysiologi excitation-kontraktion kobling kanin
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