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Chemistry

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JoVE Journal Chemistry
Optrevling Entropiske Rate Acceleration induceret af Solvent Dynamics i Membrane Enzymer
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Optrevling Entropiske Rate Acceleration induceret af Solvent Dynamics i Membrane Enzymer

Article DOI: 10.3791/53168-v • 09:42 min • January 16th, 2016
January 16th, 2016 •
1, 1
1Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology

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Kanaler til transport af vandmolekyler i enzymer indflydelse aktivt sted solvatisering og katalyse. Heri præsenterer vi en protokol for engineering af disse yderligere katalytiske motiver baseret på in silico computermodeller og eksperimenter. Dette vil øge vores forståelse af den indflydelse, opløsningsmidler dynamik på enzym katalyse.

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Kemi Enzyme katalyse termodynamik vand dynamik membranprotein kinetik overgang state teori protein engineering hydrofob effekt
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