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Journal / Bioengineering / Studying the Stoichiometry of Epidermal Growth…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
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Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

Article DOI: 10.3791/53186-v • 09:16 min • September 11th, 2015
September 11th, 2015 •
1, 1,2
1INM-Leibniz Institute for New Materials, 2Department of Physics, University of Saarland

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This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.

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Stoichiometry Epidermal Growth Factor Receptor Intact Cells Correlative Microscopy Labeling Fluorescence Microscopy Environmental Scanning Electron Microscopy (ESEM) Quantum Dots (QDs) Protein Labeling Receptor Clustering Overview Images Cellular Locations Scanning Transmission Electron Microscopy (STEM) Nanoscale Resolution Correlative Images Single Molecular Level Hydrated Intact Cell Monomer Homodimer Small Clusters
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