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JoVE Journal
Biology

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采用数字液滴PCR技术检测BRAF V600E突变的福尔马林固定石蜡包埋的参考标准细胞系
 
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采用数字液滴PCR技术检测BRAF V600E突变的福尔马林固定石蜡包埋的参考标准细胞系

Article DOI: 10.3791/53190-v 10:16 min October 8th, 2015
October 8th, 2015

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Summary

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Click here for the English version.

该视频的目标是展示如何执行从福尔马林固定的石蜡包埋(FFPE)的参考标准细胞系和数字液滴的PCR(ddPCR)分析的自动化DNA提取,以检测稀有突变在临床环境中。 FFPE样品中检测突变表明ddPCR的FFPE样品中的临床应用。

Tags

分子生物学,第104,DNA突变,BRAF,ddPCR,分子生物学,荧光,FFPE,癌症生物学
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