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Impiegando digitale Droplet PCR per identificare mutazioni BRAF V600E in inclusi in paraffina fissati in formalina Linee cella di riferimento standard

Article DOI: 10.3791/53190-v • 10:16 min • October 8th, 2015
October 8th, 2015 •
1, 3, 1, 3, 4, 2, 2, 1,2
1Research Institute of Pharmaceutical Science, Department of Pharmacy, College of Pharmacy, Seoul National University, 2The Center for Anti-Cancer CDx, N-Bio, Seoul National University, 3ABION CRO, 4ABION Inc., R&D Center

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L'obiettivo di questo video è quello di dimostrare come eseguire l'estrazione del DNA automatizzato da inclusi in paraffina linee cellulari fissate in formalina (FFPE) di riferimento standard e delle gocce digitali PCR (ddPCR) analisi per individuare mutazioni rare in un ambiente clinico. Rilevamento mutazioni nei campioni FFPE dimostra l'utilità clinica di ddPCR in campioni FFPE.

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Biologia Molecolare Numero 104 mutazione del DNA BRAF ddPCR Biologia molecolare fluorescenza FFPE Cancro biologia
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