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JoVE Journal Developmental Biology
Whole Retinal Explantaten aus Hühnerembryonen für die Elektroporation und Reagens-Behandlungen
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Whole Retinal Explantaten aus Hühnerembryonen für die Elektroporation und Reagens-Behandlungen

Article DOI: 10.3791/53202-v • 09:40 min • September 30th, 2015
September 30th, 2015 •
1, 1, 1
1Department of Neuroscience, Biomedical Center, Uppsala University

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Dieses Protokoll beschreibt ein Verfahren, um zu sezieren, experimentell zu manipulieren und Kultur ganze Retina-Explantaten aus Hühnerembryonen. Das Explantat-Kulturen sind nützlich, wenn hohe Erfolgsrate, Wirksamkeit und Reproduzierbarkeit sind notwendig, um die Auswirkungen von Plasmiden für die Elektroporation und / oder Reagenz Substanzen, dh, enzymatischer Inhibitoren zu testen.

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Entwicklungsbiologie Heft 103 Kultur Explantat Auge Inhibitoren,
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