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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

Article DOI: 10.3791/53215-v • 15:07 min • December 28th, 2015
December 28th, 2015 •
1,2, 3, 2, 1, 3, 2
1Department of Medicine, Weill Cornell Medical College, 2Institute for Computational Biomedicine, Weill Cornell Medical College, 3Department of Pathology and Laboratory Medicine, Weill Cornell Medical College

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Summary
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This protocol describes an approach to interrogate the recombined immunoglobulin heavy chain VDJ regions of lymphomas by deep-sequencing and retrieve VDJ rearrangement and somatic hypermutation status to delineate clonal architecture of individual tumor. Comparing clonal architecture between paired diagnosis and relapse samples reveals lymphoma relapse clonal evolution modes.

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VDJ-Seq Deep Sequencing Analysis Rearranged Immunoglobulin Heavy Chain Gene Clonal Evolution Patterns B Cell Lymphoma Tumor Clonality Tumorigenesis Disease Progression Clonal Composition Changes Micro-environment Treatments VDJ-seq Protocol Diffuse Large B Cell Lymphoma (DLBCL) Relapse VDJ Recombination Somatic Hypermutation (SHM) Next-Generation Sequencing (NGS) Libraries Amplified Rearranged Immunoglobulin Heavy Chain (IgH) VDJ Region Bioinformatics Pipelines IgH-VDJ Repertoire Clonal Architecture
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