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من التركيبات لبلورات - نحو تحديد هيكل β برميل الخارجي بروتينات الغشاء
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Chemistry
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JoVE Journal Chemistry
From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

من التركيبات لبلورات - نحو تحديد هيكل β برميل الخارجي بروتينات الغشاء

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09:55 min

July 04, 2016

DOI:

09:55 min
July 04, 2016

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Summary

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β-barrel outer membrane proteins (OMPs) serve many functions within the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Here, we hope to alleviate a known bottleneck in structural studies by presenting protocols for the production of β-barrel OMPs in sufficient quantities for structure determination by X-ray crystallography or NMR spectroscopy.

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