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JoVE Journal Developmental Biology
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Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Article DOI: 10.3791/53280-v • 09:16 min • December 14th, 2015
December 14th, 2015 •
1,2, 1,2
1Department of Dermatology, UCSD Stem Cell Program, University of California, San Diego, 2Department of Cellular and Molecular Medicine, UCSD Stem Cell Program, University of California, San Diego

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Summary
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The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.

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Genetically Modified Organotypic Skin Cultures Devitalized Human Dermis 3D Environment Cell-cell Contact Cell-matrix Interactions Epidermal Differentiation Stratification Program Primary Human Epidermal Keratinocytes Retroviruses Overexpressed Knocked Down Genetic Pathways Epidermal Growth Differentiation Disease Progression Gene Expression Profiling Immunostaining Chromatin Immunoprecipitations High Throughput Sequencing Skin Homeostasis
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