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JoVE Journal Neuroscience
La construction de base de cellules fluorescentes neurotransmetteur Engineered Reporters (CNiFERs) pour la détection optique des neurotransmetteurs
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La construction de base de cellules fluorescentes neurotransmetteur Engineered Reporters (CNiFERs) pour la détection optique des neurotransmetteurs

Article DOI: 10.3791/53290 • 12:48 min • May 12th, 2016
May 12th, 2016 •
1, 2, 1, 2,3, 1
1Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 2Department of Physics, University of California, San Diego, 3Section of Neurobiology, University of California, San Diego

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Nous présentons un protocole pour créer journalistes neurotransmetteur fluorescents à base de cellules modifiées (CNiFERs) pour la détection optique de la libération de neurotransmetteurs volumétrique.

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Neuroscience numéro 111 imagerie optique FSCV la dialyse la transmission de volume les neurotransmetteurs neuropeptides TPLSM biocapteur GPCR
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