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Metodi di trasferimento Proprio localizzata di cellule o DNA in precoce post-impianto di embrioni di topo
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Metodi di trasferimento Proprio localizzata di cellule o DNA in precoce post-impianto di embrioni di topo

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09:04 min

December 25, 2015

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09:04 min
December 25, 2015

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Summary

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We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.

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