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Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

9,860 Views

10:16 min

February 14, 2016

DOI:

10:16 min
February 14, 2016

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Summary

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This protocol describes an accurate, inexpensive, rapid and non-toxic method to determine the sex of Day 30 porcine embryo using PCR method after grinding an embryo into powder without phenol chloroform extraction and DNA column purification.

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