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JoVE Journal
Bioengineering

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Easy and Accurate Mechano-profiling on Micropost Arrays
 

Easy and Accurate Mechano-profiling on Micropost Arrays

Article DOI: 10.3791/53350-v 10:25 min November 17th, 2015
November 17th, 2015

Chapters

Summary

Described are protocols for quantifying mechanical interactions between adherent cells and microstructured substrates. These interactions are closely linked to essential cell behaviors including migration, proliferation, differentiation, and apoptosis. The protocols present an open-source image analysis software called MechProfiler, which enables determination of involved forces for each micropost.

Tags

Mechano-profiling Micropost Arrays Cell-substrate Interactions Flexible Microposts Force Read-out Cytoskeleton Focal Adhesion Points Post Deflection Patterns Force Per Post Commercially Available Microposts Software For Analyzing Images
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