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Multifunktionel, Mikropipette-baserede metode til indbygning og stimulering af bakteriel mekanosensitiv Ion Channels i dråbeform Interface-dobbeltlag
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Multifunktionel, Mikropipette-baserede metode til indbygning og stimulering af bakteriel mekanosensitiv Ion Channels i dråbeform Interface-dobbeltlag

Article DOI: 10.3791/53362-v • 09:54 min • November 19th, 2015
November 19th, 2015 •
1, 2, 3, 4, 5, 3, 4
1Department of Mechanical Engineering, Virginia Polytechnic Institute and State University, 2School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University, 3Department of Biology, University of Maryland, 4College of Engineering, University of Georgia, 5Department of Engineering Sciences and Mechanics, Virginia Polytechnic Institute and State University

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Bakterielle mekanosensitive kanaler kan anvendes som mechanoelectrical transducere i biomolekylære enheder. Dråbe grænseflade dobbeltlag (DIBS), celle-inspirerede byggestenene til disse enheder, udgør nye platforme at indarbejde og stimulere mekanosensitive kanaler. Her viser vi en ny mikropipette-baseret fremgangsmåde til dannelse af DIBS, tillader studiet af mekanosensitive kanaler under mekanisk stimulering.

Tags

Bioengineering bioteknik (generelt) dråbedistribution interface dobbeltlag MscL DPhPC mekanosensitive kanaler membran spænding lipid dobbeltlag biomolekylær enhed mechanoelectrical transducer DIB
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