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Multifunzionale, Metodo Micropipetta-based per la Costituzione e la stimolazione della batteriche meccanosensibili Ion Canali in Droplet Interface bilayers
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Multifunzionale, Metodo Micropipetta-based per la Costituzione e la stimolazione della batteriche meccanosensibili Ion Canali in Droplet Interface bilayers

Article DOI: 10.3791/53362-v • 09:54 min • November 19th, 2015
November 19th, 2015 •
1, 2, 3, 4, 5, 3, 4
1Department of Mechanical Engineering, Virginia Polytechnic Institute and State University, 2School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University, 3Department of Biology, University of Maryland, 4College of Engineering, University of Georgia, 5Department of Engineering Sciences and Mechanics, Virginia Polytechnic Institute and State University

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Canali meccanosensibili batteriche possono essere usati come trasduttori mechanoelectrical nei dispositivi biomolecolari. Bistrati interfaccia Droplet (DIB), cellula di ispirazione blocchi di costruzione di tali dispositivi, rappresentano nuove piattaforme per incorporare e stimolare canali meccanosensibili. Qui, dimostriamo un nuovo metodo basato micropipetta-di formare DIB, permettendo lo studio di canali meccanosensibili sotto stimolazione meccanica.

Tags

Bioingegneria Numero 105 bioingegneria (generale) gocciolina interfaccia bilayers MscL DPhPC canali meccanosensibili tensione della membrana doppio strato lipidico dispositivo biomolecolare trasduttore mechanoelectrical DIB
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