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Acqua in emulsioni olio
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Acqua in emulsioni olio: un nuovo sistema di montaggio idrosolubili proteine ​​clorofilla vincolante con pigmenti idrofobi

Article DOI: 10.3791/53410-v • 11:26 min • March 21st, 2016
March 21st, 2016 •
1, 2
1Department of Biological Chemistry, Weizmann Institute of Science, 2Migal-Galilee Research Institute

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Questo manoscritto descrive un metodo semplice e ad alta produttività per assemblare proteine ​​solubili in acqua con pigmenti idrofobiche che è basato su emulsioni acqua-in-olio. Dimostriamo l'efficacia del metodo per l'assemblaggio di clorofille nativi con quattro varianti di ricombinante solubile in acqua-clorofilla proteine ​​leganti (WSCPs) di piante Brassica espressi in E. coli.

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Chimica clorofilla solubile in acqua binding protein (WSCP) acqua-in-olio emulsione clorofilla il pigmento di assemblaggio di proteine UV-Visibile assorbanza dicroismo circolare (CD)
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