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Vand i olie Emulsioner
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Vand i olie Emulsioner: Et nyt system til samling Vandopløselige klorofyl-bindende proteiner med Hydrofobe Pigmenter

Article DOI: 10.3791/53410-v • 11:26 min • March 21st, 2016
March 21st, 2016 •
1, 2
1Department of Biological Chemistry, Weizmann Institute of Science, 2Migal-Galilee Research Institute

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Dette håndskrift beskriver en enkel og høj-throughput fremgangsmåde til samling vandopløselige proteiner med hydrofobe pigmenter, der er baseret på vand-i-olie emulsioner. Vi viser effektiviteten af fremgangsmåden til samling af native chlorophyller med fire varianter af rekombinant vandopløseligt-klorofyl proteiner (WSCPs) af Brassica planter udtrykt i E. coli.

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Kemi Vandopløseligt klorofyl bindende protein (WSCP) vand-i-olie-emulsion klorofyl pigment-proteinkonstruktionen UV-Visible absorbans cirkulær dikroisme (CD)
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