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Developmental Biology

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JoVE Journal Developmental Biology
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
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Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Article DOI: 10.3791/53426-v • 10:05 min • November 13th, 2015
November 13th, 2015 •
1, 1, 1, 1, 1, 1
1Department of Pathology and Laboratory Medicine, McAllister Heart Institute, University of North Carolina, Chapel Hill

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Summary
Automatic Translation

We describe here a protocol for the generation of iCMs using retrovirus-mediated delivery of Gata4, Tbx5 and Mef2c in a polycistronic construct. This protocol yields a relatively homogeneous population of reprogrammed cells with improved efficiency and quality and is valuable for future studies of iCM reprogramming.

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Induced Cardiomyocytes Polycistronic Construct Gata4 Mef2c Tbx5 Cardiac Fibroblasts Regenerative Medicine Heart Disease Treatment Forced Expression Defined Factors Reprogramming Efficiency In Vitro G,M,T-transduced Fibroblasts Reprogramming Mechanisms Stoichiometry MGT Vector Reprogramming Process Cardiac Fibroblast Isolation
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