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Real-time beeldvorming van Plant Cell Surface Dynamics met variabele hoek epifluorescentiemicroscopie
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Real-time beeldvorming van Plant Cell Surface Dynamics met variabele hoek epifluorescentiemicroscopie

Article DOI: 10.3791/53437-v • 06:31 min • December 12th, 2015
December 12th, 2015 •
1
1Graduate School of Frontier Sciences, The University of Tokyo

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Het doel van dit protocol is om te demonstreren hoe fluorescent-gelabelde eiwit dynamiek op plantencel oppervlakken met een variabele hoek epifluorescentiemicroscopie, waaruit blijkt knipperende stippen van GFP-gelabeld PATROL1, een membraan mensenhandel eiwit te controleren, in de cel cortex van de stomatale complex in Arabidopsis thaliana.

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Plant Biology , Celoppervlak Exocytosis fluorescerende eiwitten beeldanalyse kymograaf Membraan mensenhandel Plant celbiologie real-time beeldvorming Stomata totale interne reflectie microscopie Variable-angle epifluorescentiemicroscopie
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