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Expressão Mammalian Cell eficiente e de uma etapa de purificação extracelular glicoproteínas para a cristalização

Article DOI: 10.3791/53445-v • 07:08 min • December 23rd, 2015
December 23rd, 2015 •
*1,2,3, *2,3,4,5, 2,3,5,6,7
1Molecular Microbiology and Microbial Pathogenesis Program, Washington University School of Medicine, 2Department of Internal Medicine, Washington University School of Medicine, 3Drug Discovery Program in Pulmonary and Critical Care Medicine, Washington University School of Medicine, 4Biochemistry Program, Washington University School of Medicine, 5Center for the Investigation of Membrane Excitability Diseases, Washington University School of Medicine, 6Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 7Department of Cell Biology and Physiology, Washington University School of Medicine
* These authors contributed equally

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Bioquímica Edição 106 a expressão da proteína de mamíferos cromatografia de afinidade de níquel glicosilação cristalografia de macromoléculas
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