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Expressão Mammalian Cell eficiente e de uma etapa de purificação extracelular glicoproteínas para a cristalização
JoVE Journal
Biology
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JoVE Journal Biology
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Expressão Mammalian Cell eficiente e de uma etapa de purificação extracelular glicoproteínas para a cristalização

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07:08 min

December 23, 2015

DOI:

07:08 min
December 23, 2015

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Summary

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This is a quick, cost-efficient protocol for the production of secreted, glycosylated mammalian proteins and subsequent single-step purification with sufficient yields of homogenous protein for X-ray crystallography and other biophysical studies.

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