This is a quick, cost-efficient protocol for the production of secreted, glycosylated mammalian proteins and subsequent single-step purification with sufficient yields of homogenous protein for X-ray crystallography and other biophysical studies.
Kober, D. L., Yurtsever, Z., Brett, T. J. Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization. J. Vis. Exp. (106), e53445, doi:10.3791/53445 (2015).