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December 17, 2015
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Summary
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Flow cytometry can be utilized to assess the state of chromatin within T cells. This protocol allows scientists to interpret evidence of chromatin decondensation during T cell activation demonstrated by an increase in the mean florescence intensity (MFI) of fluorescent Histone H3 antibodies
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Cite this Article
Bingham, K. N., Lee, M. D., Rawlings, J. S. The Use of Flow Cytometry to Assess the State of Chromatin in T Cells. J. Vis. Exp. (106), e53533, doi:10.3791/53533 (2015).
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