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JoVE Journal Developmental Biology
Culturas de geração primária de fibroblastos de rato orelha e da cauda Tecidos
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Culturas de geração primária de fibroblastos de rato orelha e da cauda Tecidos

Article DOI: 10.3791/53565-v • 06:35 min • January 10th, 2016
January 10th, 2016 •
1, 1,2
1Immunology Program, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore

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Nós descrevemos um procedimento experimental simples e rápida para a geração de fibroblastos primários dos ouvidos e caudas de camundongos. O processo não requer a formação especial animal e pode ser utilizado para a geração de culturas de fibroblastos de espigas de armazenados à temperatura ambiente durante até 10 dias.

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Biologia do Desenvolvimento edição 107 Extração fibroblastos células primárias rato caudas orelhas
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