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Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
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Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Article DOI: 10.3791/53586-v • 09:16 min • October 11th, 2015
October 11th, 2015 •
1, 1
1Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia

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Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.

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Meiotic Spindle Assessment Mouse Oocytes SiRNA-mediated Silencing Chromosome Segregation Aneuploidy Microtubule Spindle Apparatus Microtubule Organizing Centers (MTOCs) MTOC-associated Proteins Meiotic Spindle Formation Meiotic Spindle Organization Oocyte Fixation Immunofluorescence Analysis
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