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Biology

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Meiotiske Spindel Vurdering i museoocytter af siRNA-medieret Silencing
 
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Meiotiske Spindel Vurdering i museoocytter af siRNA-medieret Silencing

Article DOI: 10.3791/53586-v 09:16 min October 11th, 2015
October 11th, 2015

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Summary

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Click here for the English version.

Her præsenterer vi en protokol for specifik siRNA-medieret mRNA udtømning efterfulgt af immunofluorescensanalyse at evaluere meiotisk spindel samling og organisation i museoocytter. Denne protokol er velegnet til in vitro-udtømning af udskrifter og funktionel vurdering af forskellige spindel og / eller MTOC-associerede faktorer i oocytter.

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Molekylær Biologi oocyt meiose spindel mikrotubuli kort interfererende RNA (siRNA) mikrotubuli-organisering centre (MTOCs) pericentrin tubulin kromosom
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