Journal
/
Biology
/
Meiotische Spil Assessment in Muis Eicellen door siRNA-gemedieerde silencing
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotische Spil Assessment in Muis Eicellen door siRNA-gemedieerde silencing

Nederlands

Automatically Generated
Please note that all translations are automatically generated. Click here for the English version.

11,551 Views

•

09:16 min

•

October 11, 2015

DOI:

10.3791/53586-v

DOI:
10.3791/53586-v

•

09:16 min
October 11, 2015

•

3 Views
,
1Department of Physiology and Pharmacology,College of Veterinary Medicine, University of Georgia

Summary

Automatically generated

Hier presenteren we een protocol specifieke siRNA-gemedieerde mRNA afbraak gevolgd door immunofluorescentie analyse meiotische spileenheid en organisatie evalueren muizen oöcyten. Dit protocol is geschikt voor in vitro depletie van transcripties en functionele beoordeling van verschillende spil en / of MTOC geassocieerde factoren in oöcyten.

Explore More Videos

Meiotic SpindleSiRNAChromosome SegregationAneuploidyMicrotubule Spindle ApparatusMTOCMouse OocytesImmunofluorescence Analysis

Read Article

Cite this Article

Baumann, C., Viveiros, M. M. Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing. J. Vis. Exp. (104), e53586, doi:10.3791/53586 (2015).

Download .ris file

Copy

Share Video

.

Copy