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Meiotische Spil Assessment in Muis Eicellen door siRNA-gemedieerde silencing
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Meiotische Spil Assessment in Muis Eicellen door siRNA-gemedieerde silencing

Article DOI: 10.3791/53586-v • 09:16 min • October 11th, 2015
October 11th, 2015 •
1, 1
1Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia

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Hier presenteren we een protocol specifieke siRNA-gemedieerde mRNA afbraak gevolgd door immunofluorescentie analyse meiotische spileenheid en organisatie evalueren muizen oöcyten. Dit protocol is geschikt voor in vitro depletie van transcripties en functionele beoordeling van verschillende spil en / of MTOC geassocieerde factoren in oöcyten.

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Molecular Biology eicel meiose spindel microtubuli korte interfererende RNA (siRNA) microtubule organiserende centra (MTOCs) pericentrin tubuline chromosoom
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