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Meiotischen Spindel Assessment in Maus-Oozyten von siRNA-vermittelte Silencing
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Meiotischen Spindel Assessment in Maus-Oozyten von siRNA-vermittelte Silencing

Article DOI: 10.3791/53586-v • 09:16 min • October 11th, 2015
October 11th, 2015 •
1, 1
1Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia

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Hier präsentieren wir ein Protokoll für spezifische siRNA-vermittelten mRNA-Abbau, gefolgt von Immunfluoreszenz-Analyse, um meiotische Spindel-Anordnung und Organisation in der Maus-Oozyten zu bewerten. Dieses Protokoll ist für die in vitro-Abreicherung von Transkripten und funktionale Bewertung verschiedener Spindel und / oder MTOC-assoziierten Faktoren in Oocyten.

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Molecular Biology Ausgabe 104 Eizelle Meiose Spindel Mikrotubuli short interfering RNAs (siRNA) Mikrotubuli-Organisationszentren (MTOCs) Pericentrin Tubulin-Chromosom
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