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Meiotisk Spindel Vurdering i Mouse Oocytter av siRNA-mediert lyddemping

Article DOI: 10.3791/53586 • 09:16 min
October 11th, 2015 •
1, 1
1Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia

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October 11th, 2015

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Her presenterer vi en protokoll for spesifikke siRNA-mediert mRNA uttømming fulgt av immunofluorescensanalyse å evaluere meiotisk spindelenheten og organisasjon i mus oocytter. Denne protokollen er egnet for in vitro uttømming av vitnemål og funksjonell vurdering av ulike spindel og / eller MTOC-tilknyttede faktorer i eggceller.

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