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Meiotisk Spindle Bedömning i musoocyter av siRNA-medierad Ljuddämpning
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Meiotisk Spindle Bedömning i musoocyter av siRNA-medierad Ljuddämpning

Article DOI: 10.3791/53586 • 09:16 min
October 11th, 2015 •
1, 1
1Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia

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October 11th, 2015

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Här presenterar vi ett protokoll för specifik siRNA-medierad mRNA utarmning följt av immunofluorescensanalys att utvärdera meiotisk spindelenhet och organisation i musoocyter. Detta protokoll är lämplig för in vitro utarmning av transkript och funktionell bedömning av olika spindel och / eller MTOC associerade faktorer i oocyter.

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