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JoVE Journal
Biology

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减数分裂纺锤体评估在小鼠卵母细胞的siRNA介导沉默
 
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减数分裂纺锤体评估在小鼠卵母细胞的siRNA介导沉默

Article DOI: 10.3791/53586-v 09:16 min October 11th, 2015
October 11th, 2015

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Summary

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Click here for the English version.

在这里,我们提出了一个协议,用于特定的siRNA介导的mRNA枯竭其次是免疫荧光分析来评估减数分裂纺锤体组装和组织在小鼠卵母细胞。这个协议是适合体外转录耗尽的和不同的主轴和/或微管组织中心相关因子中的卵母细胞的功能评价。

Tags

分子生物学,104期,卵母细胞,减数分裂,主轴,微管,短干扰RNA(RNA干扰),微管组织中心(MTOCs),粒周,微管蛋白,染色体
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