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Identifisering av kinesin-en Cargos Bruke Fluorescensmikroskopi
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Identifisering av kinesin-en Cargos Bruke Fluorescensmikroskopi

Article DOI: 10.3791/53632-v • 08:06 min • February 14th, 2016
February 14th, 2016 •
1
1Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai

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Her blir en protokoll presentert for å identifisere kinesin-1 last. En motorless mutant av kinesin-en tung kjede (KIF5B) aggregater i cytoplasma og induserer aggregering av sine laster. Begge aggregatene blir oppdaget etter fluorescens mikroskopi. En lignende strategi kan bli anvendt for å identifisere laster med andre motor proteiner.

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Biokjemi c-MYC kinesin-en KLC1 KIF5B kinesins myosins trafficking dominant negative fluorescerende protein fluorescens mikroskopi
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