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Journal / Bioengineering / 提高2D和3D皮肤
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Bioengineering

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JoVE Journal Bioengineering
提高2D和3D皮肤
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提高2D和3D皮肤

Article DOI: 10.3791/53642-v • 09:14 min • August 22nd, 2016
August 22nd, 2016 •
1,2, 2, 2, 1,2,3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

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我们提出了一个协议，以获得富含胞外基质蛋白的细胞来源的基质，使用大分子沟渠扫污机（MMC）。此外，我们提出该结合MMC在三维器官皮肤共培养产生，从而降低了培养时间，同时保持结构的成熟的协议。

Tags

生物工程，第114，角化细胞，成纤维细胞，大分子拥挤，2D细胞培养，三维细胞培养物，皮肤器官共培养，细胞外基质，胶原I型，IV型胶原，胶原VII型，
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