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Journal / Bioengineering / Verbetering van 2D- en 3D-Skin
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Bioengineering

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JoVE Journal Bioengineering
Verbetering van 2D- en 3D-Skin
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Verbetering van 2D- en 3D-Skin

Article DOI: 10.3791/53642-v • 09:14 min • August 22nd, 2016
August 22nd, 2016 •
1,2, 2, 2, 1,2,3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

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We presenteren een protocol om cellen afgeleide matrices rijk aan extracellulaire matrix eiwitten te verkrijgen, met behulp van macromoleculaire Crowders (MMC). Bovendien presenteren we een protocol dat MMC in 3D organotypische co-kweek huid generatie, die kweektijd vermindert behoud looptijd construct omvat.

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Bioengineering keratinocyten fibroblasten macromoleculaire crowding 2D celkweek 3D-celkweek huid organotypische co-culturen extracellulaire matrix collageen type I collageen type IV collageen type VII, dermo-epidermale junctie
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