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Journal / Bioengineering / Forbedre 2D- og 3D-Skin
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Bioengineering

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JoVE Journal Bioengineering
Forbedre 2D- og 3D-Skin
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Forbedre 2D- og 3D-Skin

Article DOI: 10.3791/53642-v • 09:14 min • August 22nd, 2016
August 22nd, 2016 •
1,2, 2, 2, 1,2,3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

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Vi presenterer en protokoll for å oppnå celle-stammer matriser rik på ekstracellulære matrix proteiner, ved hjelp av makromolekylære Crowders (MMC). I tillegg presenterer vi en protokoll som inkorporerer MMC i 3D organotypiske hud co-kultur generasjon, noe som reduserer kultur tid samtidig modenhet konstruksjon.

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Bioengineering keratinocytter fibroblaster makromolekylære samles 2D cellekultur 3D cellekultur hud organotypiske ko-kulturer ekstracellulær matrise kollagen type I kollagen type IV kollagen type VII, Dermo-epidermal junction
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