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Journal / Bioengineering / Förbättra 2D och 3D Skin
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Förbättra 2D och 3D Skin
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Förbättra 2D och 3D Skin

Article DOI: 10.3791/53642-v • 09:14 min • August 22nd, 2016
August 22nd, 2016 •
1,2, 2, 2, 1,2,3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

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Vi presenterar ett protokoll för att erhålla cell härledda matriser rik på extracellulära matrixproteiner, med hjälp av makromolekylära Crowders (MMC). Dessutom presenterar vi ett protokoll som omfattar MMC i 3D organotypic hud co-kultur generation, vilket minskar kultur tid med bibehållen löptid konstruktion.

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Bioteknik keratinocyter fibroblaster makromolekylära trängsel 2D cellodling 3D cellodling hud organotypic co-kulturer extracellulära matrisen typ kollagen I kollagen typ IV kollagen typ VII, dermo-epidermal korsning
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