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JoVE Journal
Developmental Biology

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蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎
 
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蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎

Article DOI: 10.3791/53654-v 11:25 min February 22nd, 2016
February 22nd, 2016

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

该协议提出蛋白质表达的原位分析进行定量,单细胞研究在小鼠植入前胚胎沿袭规范的方法。必要的囊胚的汇编程序,整个安装蛋白的免疫检测,在一个共焦显微镜,和核分割和图像分析的样品的成像进行描述。

Tags

发育生物学,第108,鼠标胚泡,多能性,原始内胚层,图像分析,单细胞分析,定量免疫
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