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Immunology and Infection

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JoVE Journal Immunology and Infection
Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
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Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Article DOI: 10.3791/53672-v • 09:57 min • March 17th, 2016
March 17th, 2016 •
1,2,3, 1,3, 1, 1, 1,2,3, 1,2,3, 1,2,3
1LABÉO Frank Duncombe, 2Unité de Risques Microbiens (U2RM, EA 4655), Normandy University, 3Hippolia Foundation

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Summary
Automatic Translation

Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.

Tags

QPCR Method Equid Herpesvirus-2 Diagnostics Viral Load Respiratory Fluids Diagnostic Fields Quantitative Data Interpretative Aids Sensitive Method Specific Method Rapid Method AFNOR Norme NF U47-600 Erika Hue Young Researcher Nucleic Acids Extraction Lysis Solution Ethanol Silica Column Centrifuge Wash Buffers AW1 And AW2 Elution Buffer
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