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En Vivo Cerebral Funcional Imaging Enfoque Basado en calcio bioluminiscente Indicador GFP-aequorina
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En Vivo Cerebral Funcional Imaging Enfoque Basado en calcio bioluminiscente Indicador GFP-aequorina

Article DOI: 10.3791/53705-v • 12:15 min • January 8th, 2016
January 8th, 2016 •
1,2, 2,3, 1
1Equipe: Imagerie Cérébrale Fonctionnelle et Comportements (ICFC), Institut des Neurosciences Paris-Saclay (Nero-PSI), UMR-9197, CNRS/Université Paris Sud, 2Interdisciplinary Program in Neuroscience, Graduate College, University of Iowa, 3Department of Anesthesia, Carver College of Medicine, University of Iowa

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Aquí presentamos una novela Ca 2+ -Imagenología enfoque utilizando un reportero bioluminiscente. Este método utiliza una construcción GFP-aequorina fundida que se une a Ca 2+ y emite luz, eliminando la necesidad de excitación luz. Significativamente este método permite la proyección de imagen continua de largo, el acceso a las estructuras cerebrales profundas y alta resolución temporal.

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Neurociencia Número 107 Ca reportero bioluminiscente GFP-Aequorina el cuerpo de setas P2X Drosophila actividad espontánea
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