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In vivo Funktionel Brain Imaging tilgang baseret på Bioluminescent Calcium Indikator GFP-aequorin
 
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In vivo Funktionel Brain Imaging tilgang baseret på Bioluminescent Calcium Indikator GFP-aequorin

Article DOI: 10.3791/53705
January 8th, 2016

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Summary January 8th, 2016

Please note that all translations are automatically generated.

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Her præsenterer vi en ny Ca2 + -imaging tilgang ved hjælp af en bioluminiscerende reporter. Denne fremgangsmåde anvender en kondenseret konstruktion GFP-aequorin, som binder til Ca2 + og udsender lys, hvilket eliminerer behovet for lys excitation. Markant denne metode tillader lange kontinuerlig billeddannelse, adgang til dybe hjernens strukturer og høj tidsmæssig opløsning.

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