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JoVE Journal
Biology

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一氧化氮和超氧化物歧化酶恩的人脸检测在完整的动脉内皮细胞层
 
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一氧化氮和超氧化物歧化酶恩的人脸检测在完整的动脉内皮细胞层

Article DOI: 10.3791/53718-v 08:58 min February 25th, 2016
February 25th, 2016

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Summary

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Click here for the English version.

在这篇文章中我们将介绍使用荧光染料diaminofluorescein-2双乙酸钠(DAF-2DA)和二氢乙啶(DHE)对恩面对同时检测和细胞内一氧化氮的可视化(NO)和超氧阴离子(O 2的调整相对容易的方法.- )分别在新鲜分离的肥胖小鼠模型中的完整主动脉。

Tags

分子生物学,第108,动脉,共聚焦显微镜,DHE,DAF-2DA,内皮,一氧化氮,超氧阴离子,血管生物学
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