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1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg, 2Kidney Control of Homeostasis,Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg
Chapters
- 00:05Title
- 00:52Preparing the Organ Bath System
- 02:01Isolation of Mouse Aortas
- 03:03DHE and DAF-2DA Staining
- 04:47En Face Mounting and Confocal Microscopic Imaging
- 06:42Results: eNOS-uncoupling in Obese Mice
- 07:56Conclusion
इस लेख में हम एक अनुकूलित अपेक्षाकृत आसान विधि प्रतिदीप्ति डाई एन चेहरे एक साथ पता लगाने और intracellular नाइट्रिक ऑक्साइड के दृश्य (सं) और सुपरऑक्साइड आयनों (ओ 2 के लिए diaminofluorescein -2 diacetate (DAF-2DA) और dihydroethidium (DHE) का उपयोग का वर्णन .- ) क्रमश: हौसले में एक मोटापा माउस मॉडल की बरकरार aortas अलग।
