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En Face Detection av kväveoxid och superoxid i endotelskiktet intakt artärer
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En Face Detection av kväveoxid och superoxid i endotelskiktet intakt artärer

Article DOI: 10.3791/53718-v • 08:58 min • February 25th, 2016
February 25th, 2016 •
1,2, 1,2, 2,3, 1,2, 1,2
1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg, 2Kidney Control of Homeostasis, Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg

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I den här artikeln beskriver vi en anpassad relativt enkel metod med hjälp av fluorescens färgämne diaminofluorescein-2-acetat (DAF-2DA) och dihydroethidium (DHE) för en face samtidig detektion och visualisering av intracellulär kväveoxid (NO) och superoxidanjon (O2 .- ) respektive i nyisolerade intakta aorta av en fetma musmodell.

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Molecular Biology artärer konfokalmikroskopi DHE DAF-2DA endotel kväveoxid superoxidanjon Vascular Biology
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