This content is Free Access.
Chapters
- 00:05Title
- 00:57Synthesis and Preparation of Double Stranded RNA (dsRNA)
- 02:10Preparations for dsRNA Injections
- 04:07Microinjecting Pupae with dsRNA
- 06:22Culturing and Assessing Injected Pupae
- 07:12Results: Injection Survivorship and SRPN2 Knockdown by dsRNA Injection
- 08:34Conclusion
RNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during pupal development.
