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Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment
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Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment

Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment

English

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12,185 Views

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11:19 min

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February 03, 2016

DOI:

10.3791/53741-v

DOI:
10.3791/53741-v

•

11:19 min
February 03, 2016

•

6 Views
, , ,
1Department of Anatomy,University of California, 2Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, 3Department of Cell and Developmental Biology,Sackler Faculty of Medicine

Summary

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We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.

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Cite this Article

van den Bijgaart, R. J., Kong, N., Maynard, C., Plaks, V. Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment. J. Vis. Exp. (108), e53741, doi:10.3791/53741 (2016).

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