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Immunofarvning phospho-epitoper i cilierede organer Whole Mount zebrafisk embryoer
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Immunofarvning phospho-epitoper i cilierede organer Whole Mount zebrafisk embryoer

Article DOI: 10.3791/53747-v • 08:42 min • February 19th, 2016
February 19th, 2016 •
1, 2, 1
1Department of Biology, Virginia Commonwealth University, 2Center for Human Disease Modeling, Duke University Medical Center

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Teknikker beskrives at immunfarve phospho-epitoper i hele zebrafisk embryoner og derefter foretage tofarvet fluorescerende konfokal lokalisering i cellulære strukturer så små som primær cilier. Teknikkerne til fastgørelse og billeddannelse kan definere placeringen og kinetik for udseende eller aktivering af specifikke proteiner.

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Kemi Fiksering phospho-epitoper calcium cilier mikroskopi Kupffer s vesikel nyre øre zebrafisk
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