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Immunocolorazione fosfo-epitopi in Ciliated Organi di monte intero embrioni di zebrafish
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Immunocolorazione fosfo-epitopi in Ciliated Organi di monte intero embrioni di zebrafish

Article DOI: 10.3791/53747-v • 08:42 min • February 19th, 2016
February 19th, 2016 •
1, 2, 1
1Department of Biology, Virginia Commonwealth University, 2Center for Human Disease Modeling, Duke University Medical Center

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Le tecniche sono descritte a immunostain fosfo-epitopi in interi embrioni di zebrafish e quindi condurre a due colori confocale a fluorescenza localizzazione in strutture cellulari piccoli come ciglia primarie. Le tecniche di fissaggio e di imaging possono definire la posizione e cinetica di comparsa o l'attivazione di specifiche proteine.

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Chimica Fissazione fosfo-epitopi calcio cilia microscopio di Kupffer Vesicle rene orecchio zebrafish
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