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Immunfärgning fosfor-epitoper i cilieorgans Hela Mount Zebrafish embryon
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Immunfärgning fosfor-epitoper i cilieorgans Hela Mount Zebrafish embryon

Article DOI: 10.3791/53747-v • 08:42 min • February 19th, 2016
February 19th, 2016 •
1, 2, 1
1Department of Biology, Virginia Commonwealth University, 2Center for Human Disease Modeling, Duke University Medical Center

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Tekniker beskrivs till immunostain fosfor-epitoper i hela zebrafisk embryon och sedan genomföra två-färg fluorescerande konfokal lokalisering i cellstrukturer så små som primär cilier. Teknikerna för fixering och bildbehandling kan definiera platsen och kinetik av utseende eller aktivering av specifika proteiner.

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Kemi Fixering fosfor-epitoper kalcium cilier mikroskopi Kupffer s vesikel njure öron zebrafisk
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